Primordial germ cells are capable of producing cells of the hematopoietic system in vitro

Primordial germ cells are capable of producing cells of the hematopoietic system in vitro.

The identity of the cells giving rise to the hematopoietic system in the mouse embryo are unknown. The results presented here strongly suggest that hematopoietic cells are derived from a nonhematopoietic cell population that has been previously thought to give rise to the germ cells. These cells are called primordial germ cells (PGCs) and can be recognized as large cells showing blebbing and ps...

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

generation of in-vitro spermatogonial stem cells following genetic manipulation of primordial germ-like cells

research about potential use of stem cells for the development of germ line cells in vitro had been challenged. in the present study, we reported a novel protocol consisting of cocktail growth factor addition for germ cell differentiation followed by transfection. the cells were purificated based on the expression on the cell surface of a protein. this protein is not present in normal cells of ...

Review of Differentiation and Proliferation of Primordial Germ Cells in Culture

Primordial germ cells (PGCs) are highly specialized cell population that arises from the epiblast in vivo. There are three critical steps in the life cycle of these cells: 1-Specification 2-migration and proliferation 3-prenatal and postnatal sex specific development. Specification of germ cells in epiblast occurs due to signals secreted from extraembryonic tissues. Primordial germ cells are re...

The effect of BMP4 on mouse embryonic stem cell proliferation and differentiation into primordial germ cells

Background and Aim: Artificial gamete production from stem cells is a novel strategy for treatment of infertility. Among various stem cell sources, embryonic stem cells (ESC) can be considered as an appropriate source for in vitro formation of germ cells. In this study we evaluated the effect of BMP4 on proliferation and differentiation of mouse embryonic stem cells into primordial germ cells (...